Arp2 depletion inhibits sheet-like protrusions but not linear protrusions of fibroblasts and lymphocytes
نویسندگان
چکیده
منابع مشابه
Cell Protrusions
Introduction How do cells move? There is no shortage of theories, but a definitive answer is still elusive. Here we will present some models for cell motions along with proposals for experiments to address the theories. We will restrict ourselves to the problem of cell protrusion, although the models apply to other motility phenomena as well. Different protrusion phenomena extend at different c...
متن کاملLocalization of all seven messenger RNAs for the actin-polymerization nucleator Arp2/3 complex in the protrusions of fibroblasts.
The actin-related protein 2/3 (Arp2/3) complex is a crucial actin polymerization nucleator and is localized to the leading protrusions of migrating cells. However, how the multiprotein complex is targeted to the protrusions remains unknown. Here, we demonstrate that mRNAs for the seven subunits of the Arp2/3 complex are localized to the protrusions in fibroblasts, supporting a hypothesis that t...
متن کاملGap junctions are selectively associated with interlocking ball-and-sockets but not protrusions in the lens
PURPOSE Ball-and-sockets and protrusions are specialized interlocking membrane domains between lens fibers of all species studied. Ball-and-sockets and protrusions are similar in their shape, size, and surface morphology, and are traditionally believed to play a key role in maintaining fiber-to-fiber stability. Here, we evaluate the hypothesis that ball-and-sockets and protrusions possess impor...
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Here we demonstrate that Arp2/3 regulates a transition between mesenchymal and amoeboid protrusions in MCF10A epithelial cells. Using genetic and pharmacological means, we first show Arp2/3 inhibition impairs directed cell migration. Arp2/3 inhibition results in a dramatically impaired cell adhesion, causing deficient cell attachment and spreading to ECM as well as an 8-fold decrease in nascent...
متن کاملMicrotubule-based filopodium-like protrusions form after axotomy.
The growth cone at the front of a growing neurite often has F-actin-rich structures--digitate filopodia and sheet-like veils and lamellipodia--whose protrusion advances the leading edge. Microtubules and other cytoplasmic constituents later fill the protruded area, transforming it into new neuritic length. Growth can be initiated from an axon by transecting it. We have used video-enhanced contr...
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ژورنال
عنوان ژورنال: Cell Motility and the Cytoskeleton
سال: 2008
ISSN: 0886-1544,1097-0169
DOI: 10.1002/cm.20312